ItsĮxpression increases dramatically in mice at postnatal day 3, theĪpproximate time of initiation of rhodopsin expression and outer Marker of photoreceptor identity in the developing retina. Restricted to photoreceptor and pineal cells. Is highly conserved, with 99% overall identity and 100% identity in 3 4 5 6 The available mammalianĬrx sequences (human, bovine, mouse, and rat) indicate that the protein These, Crx, is a member of the otd/Otx family of Gene cascade that controls retinal development. In recent years, a number of transcription factors haveīeen identified that appear to be important players in the regulatory Transient transfection assays, zebrafish crx appears to beĪn orthologue and functional homologue of mammalian Crx. Pattern, and ability to activate rhodopsin promoter activity in Based on molecular phylogeny, chromosomal location, expression To activate, although weakly, rhodopsin promoter activity.Ĭonclusions. Similar to mammalianĬrx, zebrafish crx interacts with neural retinal leucine zipper (Nrl) Layer, in the region occupied by bipolar cells. Nuclear layer, and in cells in the outer zone of the inner nuclear In adult zebrafish, crx is expressed by both rods and cones in the outer Situ hybridization indicate that zebrafish crx isĮxpressed in the retina and pineal gland. Linkage group LG05C, a map position consistent with the location of the Zebrafish crx maps between 49.6 and 54.5 cm from the top of Zebrafish crx is most closely related to the mammalian Crx proteins,Īnd more distantly related to the Otx proteins. Based on amino acid sequence comparisons, zebrafish crx shows 50% identity with human CRX, and 85% Radiation hybrid mapping, Northern blotĪnalysis, in situ hybridization, and transient transfection studies A zebrafish retinal cDNA library was screened with a human CRX cDNA probe. When mutated cause a variety of photoreceptor degenerations. Photoreceptors and pinealocytes, regulate photoreceptor geneĮxpression, are necessary for normal photoreceptor differentiation, and Mammalian Crx genes are expressed specifically in To isolate and characterize a zebrafish Crx homologue.
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